Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A new 68 Ga-labeled dimeric FAP ligand to advance targeted PET imaging of cancer

doi: 10.1186/s41181-026-00424-4

Figure Lengend Snippet: Representative PET/CT images of [⁶⁸Ga]Ga-DOTA-hmFAP 1 ( left ), [⁶⁸Ga]Ga-DOTA-hmFAP 2 ( middle ), and [⁶⁸Ga]Ga-DOTA-hmFAP 2 blockade ( right ) in mice bearing HEK293T FAP tumors (dashed circles, right shoulder). Without blockade, higher tumor uptake of [⁶⁸Ga]Ga-DOTA-hmFAP 2 was consistently observed at 1 h (top) and 2 h (bottom) post-injection compared to [⁶⁸Ga]Ga-DOTA-hmFAP 1 . In contrast, blockade markedly reduced tumor radioactivity of [⁶⁸Ga]Ga-DOTA-hmFAP 2 , confirming the specific binding of this dimeric radioligand

Article Snippet: Three subcutaneous tumor models were established in female athymic nude mice (6–7 weeks old; The Jackson Laboratory) using MDA-MB-231, HeLa FAP, and HEK293T FAP cells.

Techniques: Positron Emission Tomography-Computed Tomography, Injection, Radioactivity, Binding Assay

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A new 68 Ga-labeled dimeric FAP ligand to advance targeted PET imaging of cancer

doi: 10.1186/s41181-026-00424-4

Figure Lengend Snippet: Representative 3D rendering views ( left panel ) and serial coronal slices ( right panel ) of [⁶⁸Ga]Ga-DOTA-hmFAP 2 PET/MR images in a mouse bearing a xenografted HEK293T FAP tumor on the right shoulder at 1 and 2 h post-injection. Substantial focal radioactive uptake was observed in the tumor. Tomographic slices further revealed detailed organ distribution of [⁶⁸Ga]Ga-DOTA-hmFAP 2 in the liver, gastrointestinal tract, kidneys, and bladder

Article Snippet: Three subcutaneous tumor models were established in female athymic nude mice (6–7 weeks old; The Jackson Laboratory) using MDA-MB-231, HeLa FAP, and HEK293T FAP cells.

Techniques: Positron Emission Tomography-Magnetic Resonance Imaging, Injection

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A new 68 Ga-labeled dimeric FAP ligand to advance targeted PET imaging of cancer

doi: 10.1186/s41181-026-00424-4

Figure Lengend Snippet: Biodistribution (%ID/g) of [⁶⁸Ga]Ga-DOTA-hmFAP 1 , [⁶⁸Ga]Ga-DOTA-hmFAP 2 , and [⁶⁸Ga]Ga-DOTA-hmFAP 2 with unlabeled hmFAP 2 blockade in mice bearing HEK293T FAP tumors. Data were collected at the end of imaging sessions, 2–3 h post radiotracer injection. Tumor uptake of [⁶⁸Ga]Ga-DOTA-hmFAP 2 was significantly higher than that of [⁶⁸Ga]Ga-DOTA-hmFAP 1 and was markedly reduced upon co-injection with the cold ligand. Renal radioactivity was also higher for [⁶⁸Ga]Ga-DOTA-hmFAP 2 compared with [⁶⁸Ga]Ga-DOTA-hmFAP 1 . * P < 0.01 vs. ⁶⁸Ga[Ga]-DOTA-hmFAP 1 ; ‡ P < 0.01 vs. ⁶⁸Ga[Ga]-DOTA-hmFAP 2 without blockade

Article Snippet: Three subcutaneous tumor models were established in female athymic nude mice (6–7 weeks old; The Jackson Laboratory) using MDA-MB-231, HeLa FAP, and HEK293T FAP cells.

Techniques: Imaging, Injection, Radioactivity

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A new 68 Ga-labeled dimeric FAP ligand to advance targeted PET imaging of cancer

doi: 10.1186/s41181-026-00424-4

Figure Lengend Snippet: ROI analysis of PET/MR images acquired at 2–3 h after radiotracer injection in mice bearing HEK293T FAP tumors. The tumor uptake of [⁶⁸Ga]Ga-DOTA-hmFAP 2 was 3.7-fold higher than that of [⁶⁸Ga]Ga-DOTA-hmFAP 1 and was significantly reduced upon co-administration with the cold hmFAP 2 ligand. Significant differences in liver and renal radioactivity of [⁶⁸Ga]Ga-DOTA-hmFAP 2 were also observed between mice with and without blockade. * P < 0.001 vs. [⁶⁸Ga]Ga-DOTA-hmFAP 1 ; ‡ P < 0.05 vs. [⁶⁸Ga]Ga-DOTA-hmFAP 2 without blockade

Article Snippet: Three subcutaneous tumor models were established in female athymic nude mice (6–7 weeks old; The Jackson Laboratory) using MDA-MB-231, HeLa FAP, and HEK293T FAP cells.

Techniques: Positron Emission Tomography-Magnetic Resonance Imaging, Injection, Radioactivity

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A new 68 Ga-labeled dimeric FAP ligand to advance targeted PET imaging of cancer

doi: 10.1186/s41181-026-00424-4

Figure Lengend Snippet: Microphotographs (400×) of xenografted HeLa FAP ( left ), MDA-MB-231 ( middle ), and HEK293T FAP ( right ) tumor Sections. (5 μm) in nude mice stained with H&E ( top ) and anti-FAP immunohistochemistry ( bottom ). Positive FAP expression was detected in all three tumor types, with the strongest staining in HEK293T FAP tumors. MDA-MB-231 tumors exhibited relatively weaker and more scattered FAP expression

Article Snippet: Three subcutaneous tumor models were established in female athymic nude mice (6–7 weeks old; The Jackson Laboratory) using MDA-MB-231, HeLa FAP, and HEK293T FAP cells.

Techniques: Staining, Immunohistochemistry, Expressing